Journal: PLoS ONE

Article Title: A Novel Epitope from CD22 Regulates Th1 and Th17 Cell Function in Systemic Lupus Erythematosus

doi: 10.1371/journal.pone.0064572

Figure Lengend Snippet: (A) The immunofluorescence assay for the combination of different Abs to mouse CD22 molecular on B cells from MRL/lpr mice. The purified B cells were stained with anti-mouse CD19 Abs (green) and anti-B2249/B2267/B2285/KLH Abs (red) separately (magnification×400). The double staining of B cells (yellow) indicated the different binding affinity of four Abs to mouse CD22. (B) Western blotting analysis indicated the specific recognition of anti-B2249/B2267/B2285/KLH Abs for the CD22 protein (135 Kd) extracted from B cells. (C) Western blotting analysis indicated that anti-B2285 Abs could recognize the whole B cell lysate merely at CD22 protein site (135Kd), and did not bind to proteins from CD4 + T cell lysate.

Article Snippet: Briefly, purified B cells were fixed with 4% paraformaldehyde at 4°C for 30 min and then incubated with the anti-B2285 Abs (1:1000) overnight, followed by FITC-conjugated anti-mouse CD19 Abs (Biolegend, California, USA) and PE-conjugated anti-mouse CD22 (clone: 2D6, eBioscience, California, USA) for 15 min at 4°C.

Techniques: Immunofluorescence, Purification, Staining, Double Staining, Binding Assay, Western Blot

Journal: PLoS ONE

Article Title: A Novel Epitope from CD22 Regulates Th1 and Th17 Cell Function in Systemic Lupus Erythematosus

doi: 10.1371/journal.pone.0064572

Figure Lengend Snippet: (A) The flow cytometry analysis for the combination of anti-B2285 Abs to mouse B cells from MRL/lpr mice in PBS, anti-KLH Abs, anti-B2285 Abs and NB2285 (B2295 peptide neutralization Abs). (B) The analysis for the combination of anti-B2285 Abs to mouse CD4 + T cells from MRL/lpr mice by flow cytometry. (C) The detection of CD22 internalization mediated by preincubated with different Abs on viable B cells by flow cytometry. (D) The assay for the competitive binding to fixed B cells between anti-B2285 Abs and commercial anti-mouse CD22 Abs. The percentage of CD19 + CD22 + B cells was decreased obviously in B2285 group compared with those in PBS and KLH groups.

Article Snippet: Briefly, purified B cells were fixed with 4% paraformaldehyde at 4°C for 30 min and then incubated with the anti-B2285 Abs (1:1000) overnight, followed by FITC-conjugated anti-mouse CD19 Abs (Biolegend, California, USA) and PE-conjugated anti-mouse CD22 (clone: 2D6, eBioscience, California, USA) for 15 min at 4°C.

Techniques: Flow Cytometry, Neutralization, Binding Assay

Journal: Current Biology

Article Title: Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis

doi: 10.1016/j.cub.2017.07.011

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti alpha-tubulin (2D6) , Thermo Fisher Scientific , Cat# MA5-17193; RRID: AB_2538664.

Techniques: Modification, Virus, Recombinant, Mass Spectrometry, Control, Mutagenesis, Negative Control, Software

Journal: International Journal of Oncology

Article Title: PP2B and ERK1/2 regulate hyaluronan synthesis of HT168 and WM35 human melanoma cell lines

doi: 10.3892/ijo.2015.3313

Figure Lengend Snippet: The antibodies used in the experiments.

Article Snippet: Anti-RHAMM , Mouse, monoclonal, clone: 2D6 , 1:400 , 1:100 , Novocastra, Newcastle, UK NCL-CD168.

Techniques: Western Blot, Immunocytochemistry